Molecular Detection of Pathogenic Entamoeba histolytica based on Amoebapore C Gene in Fecal Samples of Camels from Babylon Province, Iraq

Abstract

The attention in the current study was payed to the identification of pathogenic Entamoebahistolytica(PEH) using molecular techniques targetedtheamoebapore C (AP-C) gene in fecal samples of camels from Babylon province, Iraq. Fifty, 50 camels, fecal samples were collected and analyzed in a laboratory using a real-time polymerase chain reaction (RT-PCR) method to detect the amplification of the AP-C gene. The results demonstrated that 28 (56%) out of 50 samples were positive for the presence of the AP-C gene. The findings showed high efficiency rate (99.7%) of the current used technique in detecting the gene in the fecal samples of camels.The present work provides interesting data about the current presence of the pathogenic Entamoebahistolytica in the feces of the tested camels from Babylon Province, Iraq.