Molecular Characterization of Malassezia spp Isolated from Human Pityriasis Versicolor

Abstract

The study was looking to isolation and identification of various species of Malassezia isolates associated
with various clinical phenomena of pityriasis versicolor by molecular Techniques the results of DNA
extraction showed that most of the isolates give just single bundle of DNA on agarose gel, and according
to PCR results, thirteen DNA samples (43.3%) were amplified after PCR program for amplification by two
sets of primers ITS1 - ITS4 3.2 and V9G - LS266, then all the thirteen isolates were subjected to DNA
sequencing which showed the following results: eight isolates identified as Malassezia furfur and five as
Malassezia globosa with the alignment bellow of each species. Malassezia furfur isolate POL.10.11.IIIA
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and
internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence .Sequence
ID: KC152898.1 Length: 848 Number of Matches: 1Range 1: 1 to 848. Phylogenetic analysis of the 13
Malassezia isolates were analyzed by MEGA 5.05 and compared with sequences of different Malassezia
species available in Gen Bank database, the data showed a clear convergence between our Malassezia
isolates from Basra patients and that of the Gen Bank database.